Biological Indicators

3M Biological Indicators for Ethylene Oxide and Steam Sterilization Processes

3M monitoring products contain highly resistant, standardized populations of Bacillus atrophaeus (Bacillus subtilis var. niger) spores for EO and Geobacillus stearothermophilus (Bacillus stearothermophilus) spores for steam. 3M biological indicators comply with pertinent industry standards such as:

• ANSI/AAMI ST59, ST21, & ST19
• ISO 11139, Parts 1, 2 & 3
• EN 866, Parts 1, 2 & 3

3M™ Attest™ Biological Indicators

• Highly resistant, standardized population of Bacillus atrophaeus) Bacillus subtilis var. niger) spores for EO or Geobacillus stearothermophilus (Bacillus stearothermophilus) spores for steam
• Conventional self-contained technology



• Simplistic culturing
• Minimizes false positives
• Cost-effective
• Routinely documented for incubation times of less than seven days

3M™ Attest™ Rapid Readout Biological Indicators

• Biological indicator with early readout capability
• Highly resistant, standardized population of Bacillus atrophaeus) Bacillus subtilis var. niger) spores for EO or Geobacillus stearothermophilus (Bacillus stearothermophilus) spores for steam
• All the benefits of self-contained technology plus results in four hours for EO or three hours for steam
• Reduced incubation time means reduced inventory levels and increased inventory velocity