Aerobic Procedure*: 3M™ Petrifilm™ Plates has an Aerobic Procedure for Lactic Acid Bacteria which utilizes the 3M™ Petrifilm™ Aerobic Count Plate supplemented with MRS (deMan, Rogosa and Sharpe) medium and a pH indicator to enumerate lactic acid bacteria in salad dressings, Ready-to-Eat meats, Ready-to-Eat salads and stainless steel surfaces.
Food processors and reference labs have demonstrated feasibility relative to their current lactic acid bacteria testing with a simple 3 step procedure: 1. Combine - MRS/pH indicator broth with food or environmental sample and place sample on the center of a 3M Petrifilm Aerobic Count Plate. 2. Incubate - The space saving design maximizes incubator space. 3. Enumerate - A red indicator dye in the plate reacts with growing bacteria allowing colony visualization. Acid producing bacteria produce a yellow zone (halo) around the colony.
*3M Petrifilm Plate performance has not been evaluated with all combinations of microbial flora, incubation conditions and food matrixes. It is the user's responsibility to determine that any test methods and results meet the user's requirements.