The 3M™ Attest™ Super Rapid Readout Biological Indicator 1491 is a self-contained biological indicator specifically designed for rapid and reliable qualification testing and routine monitoring of 270°F (132°C) and 275°F (135°C) gravity-displacement steam sterilization processes when used in conjunction with the 3M™ Attest™ Auto-reader 490. The self-contained design includes a spore carrier with spores of Geobacillus stearothermophilus and a media ampoule containing bacteriological media. The spore carrier and media ampoule are contained in a plastic vial topped with a blue cap. A chemical process indicator which changes from pink to brown upon exposure to steam is located on the top of the cap. After processing, the biological indicator is activated and incubated in the 3M™ Attest™ Auto-reader 490. A final negative reading after 30 minutes of incubation indicates an acceptable sterilization process.
For 132°C (270°F) and 135°C (275°F) 3-minute and 10-minute Gravity-Displacement Steam Sterilization cycles (Blue Cap)