Applications in the production of recombinant proteins.
Production of therapeutics and diagnostics by cell culture processes has become the cornerstone of the biotechnology industry. Cell culture systems can consist of bacterial, yeast, insect or mammalian cells, with mammalian cell culture Production becoming the most widely used method. Production of cell culture derived therapeutics begins with fermentation of the desired organism followed by purification of the cell-expressed therapeutic protein. The first step in purification involves separating cell mass from product. Separation of the cell culture fluid challenges the process engineer to select a separation method that results in maximum product yield, complies with FDA regulatory requirements, and offers acceptable economic performance.
The following table outlines 3M Purification solutions for various process steps.